吴慧凤, 宋希强, 胡美姣. 铁皮石斛促生内生真菌的筛选与鉴定[J]. 西南林业大学学报, 2011, 31(5): 45-48. DOI: 10.3969/j.issn.2095-1914.2011.05.011
引用本文: 吴慧凤, 宋希强, 胡美姣. 铁皮石斛促生内生真菌的筛选与鉴定[J]. 西南林业大学学报, 2011, 31(5): 45-48. DOI: 10.3969/j.issn.2095-1914.2011.05.011
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WU Huifeng1, SONG Xiqiang1, 2. Screening and Identification of Endophytic and Probiotic Fungi of Dendrobium catenatum[J]. Journal of Southwest Forestry University, 2011, 31(5): 45-48. DOI: 10.3969/j.issn.2095-1914.2011.05.011
Citation: WU Huifeng1, SONG Xiqiang1, 2. Screening and Identification of Endophytic and Probiotic Fungi of Dendrobium catenatum[J]. Journal of Southwest Forestry University, 2011, 31(5): 45-48. DOI: 10.3969/j.issn.2095-1914.2011.05.011
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铁皮石斛促生内生真菌的筛选与鉴定

Screening and Identification of Endophytic and Probiotic Fungi of Dendrobium catenatum

    摘要
  • 摘要: 从铁皮石斛根中分离得到37株内生真菌，经过与组培苗共生筛选，获得9株促生菌，其中3株真菌对铁皮石斛的鲜质量增长率、根数增长率和芽数的促生效果最好。采用形态学观察和ITS序列测定相结合的方法对这9株促生菌进行鉴定。经形态学观察，初步鉴定出2株，均为瘤菌根菌属。根据rDNA ITS序列分析显示，2株与Epulorhiza sp.的相似性分别为100%和96%，1株与Guignardia camelliae的相似性达100%，1株与Mycosphaerella cruenta的相似性为99%。另外5株促生真菌分类地位尚不能确定，但这5株菌株与登录号为EU482263、GQ996178、AM901831、GU566294和GU566256的序列相似性分别达96%、95%、99%、98%和100%。

     

    Abstract: 37 strains of endophytic fungi were isolated from roots of Dendrobium catenatum. Among which 9 strains were screened out to show the positive effect on the growth after being in vitro inoculated with D. catenatum seedlings. 3 strains were the most beneficial promote the growth rate of fresh biomass, the increment rate of the radical number, and the number of new buds. The 9 probiotic fungi were identified by morphological characteristics observation and ITS gene sequence analysis. 2 strains were identified to belong to genus Epulorhiza according to the morphological characteristics. Based on ribosomal DNA (rDNA) sequence analysis, 2 strains were identified as Epulorhiza sp.(the identity was 100% and 96% respectively), 1 strain was identified as Guignardia camelliae (the identity was 100%), 1 strain was identified as Mycosphaerella cruenta (the identity was 99%). The classification position of the rest 5 growthpromoting fungi were not yet determined. However, the identity of the 5 strains with the GenBank codes EU482263, GQ996178, AM901831, GU566294 and GU566256 was 96%, 95%, 99%, 98% and 100%, respectively.

     

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