康柳, 熊智, 杜健伟, 刘绍雄, 朱丽丽. 小蠹虫肠道中纤维素降解菌的分离及其产酶性质[J]. 西南林业大学学报, 2012, 32(2): 7-10. DOI: 10.3969/j.issn.2095-1914.2012.02.002
引用本文: 康柳, 熊智, 杜健伟, 刘绍雄, 朱丽丽. 小蠹虫肠道中纤维素降解菌的分离及其产酶性质[J]. 西南林业大学学报, 2012, 32(2): 7-10. DOI: 10.3969/j.issn.2095-1914.2012.02.002
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KANG Liu, XIONG Zhi, DU Jianwei, LIU Shaoxiong, ZHU Lili. Isolation of Cellulose Degradating Bacteria from Bark BeetlesIntestine and Enzyme Production Properties[J]. Journal of Southwest Forestry University, 2012, 32(2): 7-10. DOI: 10.3969/j.issn.2095-1914.2012.02.002
Citation: KANG Liu, XIONG Zhi, DU Jianwei, LIU Shaoxiong, ZHU Lili. Isolation of Cellulose Degradating Bacteria from Bark BeetlesIntestine and Enzyme Production Properties[J]. Journal of Southwest Forestry University, 2012, 32(2): 7-10. DOI: 10.3969/j.issn.2095-1914.2012.02.002
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小蠹虫肠道中纤维素降解菌的分离及其产酶性质

Isolation of Cellulose Degradating Bacteria from Bark BeetlesIntestine and Enzyme Production Properties

    摘要
  • 摘要: 从小蠹虫幼虫肠道中筛选分离出降解纤维素的菌株5株，通过杜氏纤维素选择性培养基，以滤纸、脱脂棉和羧甲基纤维素钠为底物，测定菌株所产纤维素酶的活力。结果表明：酶活最强的为HS-11-5号菌株，最大酶活32.62U/mL；此菌株的最佳产酶条件为：培养发酵时间50h,培养所需最佳氮源为硫酸铵,培养最佳pH 7.5~8.5,培养最适温度28~30℃,最佳瓶装量为占培养瓶容积的12%~16％。

     

    Abstract: Five strains of cellulose degrading bacteria were isolated from the intestine of young bark beetle, and the activity of cellulase generated by these strains was determined by Dus cellulose selective medium on filter paper or absorbent cotton with CMC-Na as fermentation substrate. The results showed that HS-11-5 was the strain with the highest cellulase activity (32.62 U/mL), whose optimum cellulase producing conditions were as follows: 50h of fermentation; (NH4)2SO4 as nitrogen source; under pH value of 7.5-8.5 at 28-30℃; and 12%-16% as the medium loading proportion.

     

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