Abstract: By means of constructing the structures by spectroscopic methods and comparing with literature, nine compounds were isolated from petroleum ether and ethyl acetate extraction of Sulcaria sulcata, using different chromatography isolation methods. The nine compounds were as follows: ethyl haematommate (1)； 5α,8α-epidioxy-(22E,24R)- ergosta- 6,9(11), 22-trien-3β-ol (2)； 2-methoxypsoromic acid (3)； olivetoric acid (4)； methyl β-orsellinatel (5)； virensic acid (6)； atranorin (7)； atranol (8)； and methyl haematommate (9). One pure compound was isolated from methanol extraction of S. sulcata f. vulpinoides, which was identified as psoromic acid (10)； Taking the compounds isolated from the two different species as control, the typical chemical composition of these compounds and the main differences in chemical composition of the compounds extracted from S. sulcata and S. sulcata f. vulpinoides were determined by testing 10 samples individually with TLC methodology.