蓖麻LPAAT基因序列的SNPs及与油脂的相关性

Correlation Between Single Nucleotide Polymorphisms of LPAAT Gene Sequence and Oil Content of Ricinus communis

摘要: LPAAT对蓖麻储存油脂（triacylglycerols, TAG）的合成调控具有重要作用。为了研究蓖麻LPAAT的多态性，参考GenBank中编码蓖麻LPAAT的基因组序列设计引物，对来自32个不同地区蓖麻种质的LPAAT进行测序，获得长约804bp的基因组序列。多态分析表明：在804bp的区间内共发现2个SNP和3个InDel, SNP频率为1/161bp，多样性指数Pi为000067。在外显子区域，有1个SNP和2个InDel，其中2个为同义突变，1个为错意突变。结果表明，基因LPAAT的exon10与种子油脂含量有明显相关性。

Abstract: The LPAAT gene of Ricinus communis plays an important role in synthesizing and regulating the fatty acid (triacylglycerols, TAG). In order to study the diversity of LPAAT gene in R. communis, the LPAAT sequencing of the R. communis germplasm collected from 32 different areas was conducted by referring the LPAAT gene sequencing primers of R. communis in the GenBank, and a genomic sequence with 804bp was obtained. The results from the diversity analyses showed that there were 2 SNPs and 3 InDels among the 804bp sequence, the SNP frequency was 1/161bp, and the nucleotide diversity index (Pi) was 0.00067. There were 1 SNP and 2 InDels located at exon, two of them were nonsense mutations; the other one was missense mutation. The results showed that the exon 10 of LPAAT gene was related to the oil content of R. communis.