Abstract:
The enriched microsatellite library of Tomicus brevipilosus (Eggers) was constructed using biotinmagnetic bead enrichment method. One hundred clones randomly chosen from the library were sequenced. The results showed that 91% of microsatellite loci were obtained. Among which the microsatellite sequences with more than 10 times of alkaline base repetitions accounted for 36%, and the highest alkaline base repetition was 63 times. From these microsatellites, 47.3% (about 43 repeat motifs) were perfect, 24.2% (about 22 repeat motifs) were imperfect, and 16.5% (about 15 repeat motifs) were compound. The results indicated that the enriched microsatellite library was of highquality, in which the proportion of perfect microsatellites types was higher than that of the imperfect and compound types, which was in accordance with the feature of microsatellite sequences of other eukaryote.