王东升, 周继磊, 解荷锋, 邢世岩, 李际红, 王玮, 孙百友. 刺槐无性系遗传多样性的AFLP分析[J]. 西南林业大学学报, 2012, 32(6): 25-29. DOI: 10.3969/j.issn.2095-1914.2012.06.005
引用本文: 王东升, 周继磊, 解荷锋, 邢世岩, 李际红, 王玮, 孙百友. 刺槐无性系遗传多样性的AFLP分析[J]. 西南林业大学学报, 2012, 32(6): 25-29. DOI: 10.3969/j.issn.2095-1914.2012.06.005
shu
WANG Dongsheng1, ZHOU Jilei2, XIE Hefeng2, XING Shiyan1, LI Jihong1, WANG Wei2, SUN Baiyou3. AFLP Genetic Diversity Analysis of Robinia pseudoacacia Clones[J]. Journal of Southwest Forestry University, 2012, 32(6): 25-29. DOI: 10.3969/j.issn.2095-1914.2012.06.005
Citation: WANG Dongsheng1, ZHOU Jilei2, XIE Hefeng2, XING Shiyan1, LI Jihong1, WANG Wei2, SUN Baiyou3. AFLP Genetic Diversity Analysis of Robinia pseudoacacia Clones[J]. Journal of Southwest Forestry University, 2012, 32(6): 25-29. DOI: 10.3969/j.issn.2095-1914.2012.06.005
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刺槐无性系遗传多样性的AFLP分析

AFLP Genetic Diversity Analysis of Robinia pseudoacacia Clones

    摘要
  • 摘要: 采用AFLP分子标记技术对53个刺槐无性系进行遗传多样性和亲缘关系分析。结果显示：8对引物共检测出1230条谱带，其中有1218条为多态带，占总谱带的9902%；特异性条带有203条，其中缺失带为11条，通过特异性条带可鉴别83%的刺槐无性系；聚类分析将刺槐种质分成5组，来源相同的无性系并未严格聚在一起，未形成明显的地理变异模式。

     

    Abstract: The genetic diversity of 53 Robinia pseudoacacia clones were tested by AFLP and the parental relationship among the clones was analyzed. Totally 1230 fragments were produced with 8 pairs of primers, 1218 of the fragments were polymorphic, the polymorphism percentage was 99.02%. There were 203 specific bands and 11 absent bands by which 83% of the R. pseudoacacia clones could be identified. The germplasm of the 53 R. pseudoacacia clones was clustered into 5 groups with UPGMA, and the clones with the same origins were not always clustered into the same group, indicating that there were no obvious geographic variation modes formed in the studied R. pseudoacacia clones.

     

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