Abstract: Essential oil was extracted respectively from the fresh flowers, fresh leaves, dried flowers and dried leaves of Magnolia denudata by using steam distillation method, the extraction rate of essential oil was 0.21%, 054%, 0.27% and 0.55% respectively. The chemical compositions of the essential oil were analyzed by gas chromatographymass spectrometry (GCMS), and the results showed that the principal constituents of the essential oil were terpenes and related oxidized compounds. Among which linalool content was over 60%, followed by caryphylene, selinene, 4ally1,2dimethoxy benzene, neroidol, caryophylene oxide, and taumuurolol. The relative content of the major constituents isolated from essential oil extracted from the fresh flowers, dried flowers, fresh leaves, and dried leaves of M. denudata was 91.08 %, 91.28%, 94.67 % and 91.28%, respectively. Totally 58 major constituents were identified from the essential oil extracted from the above 4 types of raw materials, 50, 49, 48 and 40 constituents were respectively identified from the essential oil extracted from fresh flowers, dried flowers, fresh leaves and dried leaves. Although 34 constituents were commonly contained in the different types of raw materials, there were differences in the contents of each constituent among the raw materials.