Abstract: Ulmus densa and Fraxinus rhynchophylla were used to study the change of cell membrane permeability, MDA content, several related enzymes (CAT, SOD, POD) activity and root vitality of Ulmus densa and Fraxinus rhynchophylla seedlings after being offseason transplanted under different treatments (shading with liquid soaking, shading without liquid soaking, liquid soaking with shading, and the control without shading nor liquid soaking) was studied to understand the physiological restoration process. The results showed that the vitality of seedling root system increased significantly, while the cell membrane permeability, MDA content and CAT, SOD, POD enzyme activity of the two tree species changed in different ways along with the time duration increment after transplanting. The seedling growth was most severely inhibited around 12 days after being transplanted, and the physiological status was gradually recovered to be normal 30 days after transplanting. The results indicated that both Ulmus densa and Fraxinus rhynchophylla were well adaptive to offseason transplanting with good recovery in physiological indexes.