Abstract: In order to determine soil fertility in the main planting regions of Eucalyptus spp., the physical and chemical properties of E. spp. plantation soil in six counties of the southern Fujian Province were analyzed. The results showed that the E. spp. plantation soil was acid in the major planting regions. According to the classification standard of soil nutrition in China, the contents of total N, total K and organic matter in the E. spp. plantation soil was at the medium level, the content of total P and the available nutrients were at low level, while the available P was in severe deficiency, which could be a limiting factor for the growth of Eucalyptus spp.. The physical properties of the soil in the major E. spp. planting regions were at the average level, where the soil was rather compact with poor air ventilation and water permeability. Among the six counties in Southern Fujian Province, the soil fertility condition was relatively poor in Yunxiao County, and little better in Changtai County and Nanjing County. The mean fertility of E. spp. plantation soil in southern Fujian Province generally belonged to the medium to poor level, which should be ameliorated by artificial fertilization.