Abstract: 10 strains of eucalyptus endophytic fungi were inoculated into the seedlings of Eucalyptus urophylla × Eucalyptus grandis under low phosphorus stress by irrigating with the fungal solution (11 treatments added control treatment), and the experiment with low phosphorus stress was carried 30 days later. The content of soluble protein was measured 15d, 30d and 45d consequently after the inoculation, and the chlorophyll content of the seedling leaves and the chemical properties of soil were measured 3 months later. Compared to the CK group, the results showed that the content of soluble protein of the seedlings inoculated with strain G, strain E and strain J under normal phosphorus supply was increased by 2782％, 2308％ and 676％; Under No. 1 low phosphorus stress condition the content of soluble protein of the seedlings inoculated with strain G and strain J was 524％ and 1.00％ higher than that of the CK Under No. 2 low phosphorus stress condition, the content of soluble protein of seedlings inoculated with strain J, strain E and strain G was 2762％, 22.52％ and 21.95％ higher than that of the CK. The total chlorophyll content of the seedlings inoculated with strain J, strain E and strain G was 4067％, 3660％ and 32.69％ higher than that of the CK. The soil pH value that growing the seedlings inoculated with strain J, strain E and strain G was 507％, 404％ and 1.80％ lower than the CK, whereas the content of soil phosphatase that growing the seedlings inoculated with strain J, strain E and strain G was 94.50％, 88.83％ and 88.83％ higher than that of the CK Comprehensively speaking, the fungal strain E, strain G and strain J could significantly promote the contents of soluble protein and chlorophyll of Eucalyptus urophylla × Eucalyptus grandis seedlings, could increase the activity of phosphatase of the soil and improve the phosphorus absorption of the Eucalyptus urophylla × Eucalyptus grandis seedlings.