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金环胡蜂和黄脚胡蜂表皮化学成分差异分析
Difference Analysis of the Chemical Composition of the Cuticular of Vespa Mandarinia and V. Velutina
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摘要: 通过诱捕法收集金环胡蜂和黄脚胡蜂2种活体昆虫,利用气相色谱−质谱联用技术,分析2种胡蜂表皮化合物的主要成分。结果表明:从2种胡蜂表皮中共鉴定出32种化合物,以烃类、羧酸类和醇类为主,占测定化合物种类数量的70.97%;2种胡蜂表皮化合物的种类和含量均有差异,金环胡蜂表皮中相对含量最多的5种化合物为13−甲基二十七烷(32.31%)、2−甲基二十四烷(23.53%)、三十二烷(14.61%)、(E)−9−十八烯酸(12.80%)、胆固醇(5.70%),黄脚胡蜂表皮中相对含量最多的5种化合物为二十烷(22.45%)、亚磷酸三(2,4−二叔丁基苯基)酯(19.13%)、三十二烷(17.55%)、(E)−9−十八烯酸(15.66%)、13−甲基二十七烷(5.52%);2种胡蜂表皮相对含量最高的5种化合物中,2−甲基二十四烷只存在于金环胡蜂中,三十二烷和(E)−9−十八烯酸在2种胡蜂表皮中相对含量接近,二十烷、13−甲基二十七烷、亚磷酸三(2,4−二叔丁基苯基)酯在2种胡蜂表皮中相对含量差异较大。对2种胡蜂表皮化合物的研究有助于进一步揭示其生活习性和行为特征,可为更好开展虫害防治和绿色防控提供参考。Abstract: The Vespa mandarinia and V. velutina were collected using trapping methods, and the main components of the cuticular compounds of the 2 wasps were analyzed with gas chromatography-mass spectrometry. The results showed that a total of 32 compounds were identified from the cuticular of the 2 wasps, dominated by hydrocarbons, carboxylic acids and alcohols, which accounted for 70.97% of the determined compound species. The types and contents of cuticular compounds differed between the 2 wasp species. The 5 most abundant compounds in V. mandarinia were 13-Methylheptacosane(32.31%), 2-Methyltetracosane(23.53%), Dotriacontane(14.61%), (E)-9-Octadecenoic acid(12.80%), and Cholesterol(5.70%), while the 5 most abundant compounds in V. velutina were Eicosane(22.45%), Tris(2,4-ditert-butylphenyl) phosphite(19.13%), Dotriacontane (17.55%), (E)-9-Octadecenoic acid(15.66%), and 13-Methylheptacosane(5.52%). Of the 5 compounds with the highest relative amounts in the 2 wasps, 2-Methyltetracosane was present only in the V. Mandarinia. The relative contents of tridecane and (E)-9-Octadecenoic acid were relatively close in the cuticular of the 2 wasps, while the relative contents of Eicosane, 13-Methyloctadecanes, Tris(2,4-ditert-butylphenyl) phosphite had large variations in the cuticular of the 2 wasps. The study of the cuticular compounds of the 2 wasp species can help to further reveal their habits and behavioural characteristics, with a view to providing a reference for better pest control and green prevention and control.