Abstract: The petroleum ether phase, ether phase, ethyl acetate phase and methanol phase were separated from the extract of seed embryo and seed shells of Celtis sinensis seeds by systematic solvent method, and the biological activity of these 5 phases was detected by the germination test of cabbage seeds. At the same time, gas chromatography-mass spectrometry(GC-MS) combined with bioassay, were used to explore the relationship between the inhibitors and seed dormancy. The results showed that each separated phase of C. sinensis seeds had a certain inhibitory effect on the germination of cabbage seeds, among which ethyl acetate phase and ether phase were the strongest, followed by methanol phase and petroleum ether phase, respectively. Combined with the results of GC-MS and biological detection, it was found that the main components of endogenous inhibitors were organic acids: acetic acid, palmitic acid, and ester substances: dimethyl phthalate. And some benzene substances: butylated hydroxytoluene, may also have an inhibitory effect.